Effect of glucose on appearance of plasma-clotting accelerator activity, lactescence, and plasma lipids in fat-fed dogs.

The influence of glucose, given orally and intravenously, on the acceleration of plasma dotting and appearance of lipemia following the feeding of peanut oil was determined in dogs. Oral glucose blocked or masked the appearance of plasma laetescence and clotting accelerator activity, whereas intravenous administration tended to delay their appearance. In other experiments heparin abolished or reduced plasma lactescence without modifying clotting accelerator activity induced by fat feeding. T HE ingestion of a high fat meal is usually accompanied by hyperlipemia and an increase in the coagulability of the blood when measured by in vitro tests. 1 " 9 There have been several reports that carbohydrate reduces the degree of lipemia caused by the iugestion of fat. In 1918 Bang 10 fed bread with butter or olive oil to dogs and observed that the hyperlipemia was lass than that seen when the fat alone was given. Roney and Ching 11 measured total plasma fatty acids in dogs that were fed either olive oil alone or olive oil pins glucose, and concluded that the "glucose inhibited alimentary lipemia." Al-brink et al. 12> 13 have reported that in human subjects the usual rise in serum triglycerides was "diminished or absent" when 120 Gm. of extra glucose was fed with a 60 Gm. fat breakfast, and that "the lactescence was less apparent" under these conditions. AValdron et al. s showed that the acceleration in whole blood clotting time induced by fat feeding was less when either lactose or sucrose was fed simultaneously with the fat. The present report is concerned with the effects observed in dogs following orally and parenterally administered glucose, with and without fat feeding with respect to (1) appearance of coagulation accelerator activity in plasma treated with barium sulfate," (2) plasma lactescence, and (3) changes in plasma concentrations of total lipids, phospho-lipids and glucose. METHODS Accelerator Activity (AA). Nine ml. of blood were carefully drawn into a heat-sterilized syringe containing 1 ml. of 1.34 per cent sodium oxalate solution and gently mixed. To the plasma was added barium sulfate powder, 100 mg./inl., mixed 1 niin., incubated at 37 C. for 10 min., centrifuged lit 2,200 r.p.m. for 10 min., and the supernatant recentrifuged. Clotting times were determined lifter the treated plasma was combined with equal volumes (0.1 ml.) of a normal "substrate" plasmu (previously diluted 1:8 with saline), 1:10,000 Russell viper venom solution ("Stypven" Bur-roughs-Wellcome and Co.), and 0.025 M …